@article{Regecová_Výrostková_Zigo_Pipová_Jevinová_Demjanová_2021, title={IDENTIFICATION OF STAPHYLOCOCCUS SPP. ISOLATED FROM FOOD BY TWO METHODS}, volume={10}, url={https://office2.jmbfs.org/index.php/JMBFS/article/view/3110}, DOI={10.15414/jmbfs.2021.10.4.546-552}, abstractNote={<p>One hundred and ninety-three staphylococcal strains were isolated from samples of alaska pollock meat (<em>Theragra chalcogramma</em>), atlantic mackerel meat (<em>Scomber scombrus</em>),  atlantic herring meat (<em>Clupea harengus</em>), and femoral muscle samples of the wild pheasants (<em>Phasianus colchicus</em>), wild rabbits (<em>Oryctolagus cuniculus</em>) and bryndza cheese. Phenotypic manifestations typical of individual staphylococcal species were detected in these isolates. Species identification was also performed by matrix-assisted-laser-desorption-ionization-mass-spectrometry on the basis of which 5 species of staphylococci were determined: <em>S. aureus, S. epidermidis, S. capitis, S. haemolyticus, S. warneri.</em> Subsequently, the presence of the <em>16S rDNA</em> gene was confirmed by PCR assay; a specific sequence for <em>S. aureus</em> species; putative transcriptional regulator gene <em>serp0107</em>, a specific sequence of the <em>sodA</em> gene was used to identify <em>S. epidermidis</em> and to identify <em>S. warneri, S. haemolyticus, S. capitis</em> species. When comparing the results of identification by PCR assay and matrix-assisted-laser-desorption-ionization-mass-spectrometry, the identification at the genus level was identical in all cases (100%). However, the discrepancies in results were confirmed at the species level, where congruence for identification of isolates by PCR assay and matrix-assisted-laser-desorption-ionization-mass-spectrometry was only 80%. For these isolates, the score value ranged from 1.953 to 2.564.</p>}, number={4}, journal={Journal of microbiology, biotechnology and food sciences}, author={Regecová, Ivana and Výrostková, Jana and Zigo, FrantiÅ¡ek and Pipová, Monika and Jevinová, Pavlina and Demjanová, Soňa}, year={2021}, month={Feb.}, pages={546–552} }