@article{Jamalludeen_Shakir_2022, title={IN VITRO EFFECT OF NEWLY BACTERIOPHAGES ISOLATES AGAINST ESCHERICHIA COLI SEROGROUPS COLLECTED FROM THE LOCAL HOSPITAL LABORATORY; THEIR SPECIFIC CHARACTERIZATION AND IDENTIFICATION}, volume={11}, url={https://office2.jmbfs.org/index.php/JMBFS/article/view/5478}, DOI={10.55251/jmbfs.5478}, abstractNote={<p>The aim of this study is to isolate a new bacteriophage with lytic effect against several serogroups of <em>Escherichia coli</em> that were isolated from different samples submitted to the local hospital laboratory. These serogroups are the main causative agents of bloodstream and urinary tract infections (UTIs) among Gram-negative bacteria. Three isolates of <em>E. coli</em> were used as a host to isolate phages from a hospital wastewater treatment plant. Eight phages were isolated and only three of them (EC-BSR1, EC-BSR2, and EC-BSR3) were considered for further characterization and identification. The three phages appear to belong to <em>Myoviridae</em> characterized by icosahedral heads, necks and contractile tails with tail fibers. Phage EC-BSR1 and EC-BSR2 had genome sizes of 67.06 and 68.04 kb, respectively. All three phages were lysed 100% of <em>E. coli</em> serogroups that was tested <em>in vitro</em> and 42.7%, 61.7% and 44.7% <em>in vitro</em> lyses of ECOR collection reference. Phages were resistant to pH from 5 to 9, and phage EC-BSR3 appeared to be more resistant than the other two phages to an acidic and alkaline environment. These phages have the pattern of homologous DNA fragments after being digested with <em>Acc</em>1 and <em>Eco</em>R1 restriction enzymes. It was concluded that these phages are highly coli lytic for the <em>E. coli</em> serogroups isolates.</p>}, number={6}, journal={Journal of microbiology, biotechnology and food sciences}, author={Jamalludeen, Nidham and Shakir, Dania}, year={2022}, month={Jun.}, pages={e5478} }