TY - JOUR AU - Loc, Nguyen Hoang AU - Sinh, Duong Duc Hoang AU - Tue, Nguyen Hoang AU - Huy, Nguyen Duc AU - Ngoc Thi, Tran Vu AU - Ha Thanh, Le Thi PY - 2020/12/01 Y2 - 2024/03/29 TI - HETEROLOGOUS EXPRESSION OF A GENE ENCODING BIPHENYL 2,3-DIOXYGENASE FROM BURKHOLDERIA CEPACIA IN ESCHERICHIA COLI JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 10 IS - 3 SE - Biotechnology DO - 10.15414/jmbfs.2020.10.3.490-492 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/1642 SP - 490-492 AB - <p>Biphenyl 2,3-dioxygenase (BphA) is an enzyme that participates in initiation step of the pathway of biphenyl degradation in bacteria. Biphenyl is an aromatic hydrocarbon including two benzene rings that causes a serious environmental pollution. This report presents the preliminary results on the cloning and expression of <em>BphA</em> gene of <em>B. cepacia </em>strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam. The coding DNA sequences of <em>BphA</em> genes are 897 bp long and 99% identical. Recombinant BphA enzyme (33 kDa) has strongly expressed in <em>E. coli</em> M15 after induction of 0.5 mM IPTG for 4 h at 37ºC.</p> ER -