TY - JOUR AU - Naik, Milind Mohan AU - Naik-Samant, Sanika AU - Salkar, Komal AU - Manerikar, Veda PY - 2021/12/01 Y2 - 2024/03/29 TI - LISTERIA MONOCYTOGENES STRAIN BN3 RESPONDS TO ACYL HOMOSERINE LACTONE (AHL) BY EXPRESSION OF VIRULENCE GENE (HLYA) AND GENE RESPONSIBLE FOR BIOFILM (SRTA) JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 11 IS - 3 SE - Microbiology DO - 10.15414/jmbfs.1846 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/1846 SP - e1846 AB - <p><em>Listeria monocytogenes, </em>a foodborne pathogen and well known causative agent of listeriosis in humans that is also known to form biofilm on utensils used in the dairy industry is well studied for peptide-based Quorum sensing system (<em>agr </em>QS system). In the present study, we are reporting <em>L. monocytogenes </em>strain BN3 to respond to an acyl-homoserine lactone (AHL) by expression of virulence gene (<em>hlyA</em>) and the gene responsible for biofilm formation (<em>srtA</em>) using Real-Time PCR technology. It revealed that in <em>L. monocytogenes,</em><em> hlyA</em> gene (encodes listeriolysin O) expression increased 0.7-fold in response to 500 nM AHL (C4-HSL treated) as compared to control. Also, in response to 500 nM AHL, (C4-HSL treated) strain BN3 showed 27-fold up-regulation of <em>srtA</em> gene (encodes enzyme sortase) as compared to control. Our study confirmed the cross-talk between Gram-positive and Gram-negative bacteria since<em> L. monocytogenes</em> strain BN3 responds to C4-HSL, which is normally produced by Gram-negative bacteria. Here, we suggest targeting both <em>agr</em> based QS and AHL based QS system together in <em>L. monocytogenes</em> to tackle biofilm formed on milk cans in dairy industries and when treating listeria infections.</p> ER -