TY - JOUR AU - Singh, Naresh AU - Naik, Bindu AU - Kumar, Vijay AU - Kumar, Vivek AU - Gupta, Sanjay PY - 2021/02/01 Y2 - 2024/03/28 TI - ACTINOBACTERIAL PIGMENT ASSISTED SYNTHESIS OF NANOPARTICLES AND ITS BIOLOGICAL ACTIVITY: NANOPARTICLES AND ITS BIOLOGICAL ACTIVITY JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 10 IS - 4 SE - Microbiology DO - 10.15414/jmbfs.2021.10.4.604-608 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/2302 SP - 604-608 AB - <p>Recently, the green synthesis of nanoparticles has gained considerable attention due to its benefits such as cost efficiency, simplicity, eco-friendly nature, biocompatibility and broad applications over conventional chemical and physical techniques. In this context twenty actinobacteria were isolated from the rhizospheric soil of wild carrot and screened for their pigment producing ability. These isolates belong to the genus <em>Streptomyces</em> (58%), followed by <em>Streptosporangium</em> sp.  (19%), <em>Nocardia</em> sp. (11%), <em>Actinomadura</em> sp. (8%), and <em>Micromonospora</em> sp. (4%). The most promising isolate (NS-05) producing the pink pigment has been taken for the synthesis of silver nanoparticles. The isolate NS-05 was identified as <em>Streptomyces</em> sp. based on cultural characteristics and 16S rDNA sequence analysis. It was most closely related with type strain <em>Streptomyces</em> <em>fulvissimus</em> DSM 40593<sup>T</sup>, <em>S. microflavus</em> NBRC13062<sup>T</sup>,<em> S. setonii</em> NRRL ISP-5322<sup>T</sup>,<em> S. anulatus</em> RRL B-2000<sup>T</sup> with a sequence similarity of 95.6% which shows that it may belong to novel species of <em>Streptomyces</em>.  The bio-pigment assisted synthesized nanoparticles were characterized using UV-Vis, FTIR and Scanning electron microscopy studies. The average size of synthesized silver nanoparticles was 42.5nm and has λ max at 433 nm.  The synthesized nanoparticles showed promising activity against major pathogens like<em> Staphylococcus</em> <em>aureus</em> MTCC 2940, <em>Bacillus</em> <em>subtilis</em> MTCC 441<em> Salmonella typhi, Proteus vulgaris </em>MTCC 6380<em>, Escherichia coli </em>MTCC 739.  The findings of present research are promising, and this pigment can also be used for the green synthesis of other nanoparticles.</p> ER -