TY - JOUR AU - Nkanang, Abigail Johnny AU - Antai , Sylvester AU - Asitok, Atim PY - 2020/12/01 Y2 - 2024/03/28 TI - CRUDE OIL TOXICITY TOLERANCE OF HYDROCARBONOCLASTIC STRAIN OF Citrobacter amalonaticus -Y2ESW1 ISOLATED FROM ESTUARINE SEDIMENT IN THE NIGER DELTA OF NIGERIA JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 10 IS - 3 SE - Microbiology DO - 10.15414/jmbfs.2020.10.3.413-417 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/294 SP - 413-417 AB - <p>Crude oil toxicity possess a major threat to the aquatic ecosystem in the Niger Delta. This study investigated the   inherent tolerability potential of hydrocarbon degrading strain of <em>Citrobacter amalonaticus</em>  - Y<sub>2</sub>ESWS<sub>1</sub>  to crude  oil toxicity.  Sediment samples were obtained from selected locations and subjected to   bacteriological analysis using standard methods. Bacterial identification result revealed taxonomic group of hydrocarbon utilizing bacteria species including <em>Citrobacter amalonaticus</em> strain Y<sub>2</sub>ESWS<sub>1</sub>. Toxicity testing revealed that the toxicant concentration and exposure time were key variables that mediated toxicity. A second-order polynomial regression model revealed significant (<em>P</em>&lt;0.05, 0.01; <em>R</em><sup>2</sup>=0.9852) relationship between exposure time and bacterial growth at 20% crude oil. <em>Citrobacter amalonaticus</em> strain - Y<sub>2</sub>ESWS<sub>1</sub> was tolerant to crude oil toxicant concentrations as was evidenced in its prolonged acclimation periods. In this study the toxicity of pollutants to bacteria cultures (<em>Citrobacter amalonaticus</em> strain Y<sub>2</sub>ESW<sub>1</sub>,) was highly dependent on the concentration of the toxicants. The bacterial proliferation was hindered by 20% toxicant. It is a pointer to its poor activity when exposed to hydrocarbon however it can be used in a consortium with other microbes including potent degraders to achieve enhanced degradation of contaminated site.</p> ER -