TY - JOUR AU - Gusti Ningrum, Siti AU - Wibawan, I Wayan Teguh PY - 2021/06/01 Y2 - 2024/03/29 TI - EVALUATION OF THE DOMINANT PROTEIN FROM WHOLE-CELL HEATING METHOD USING MALDI-TOF/TOF MASS SPECTROMETRY JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 10 IS - 6 SE - Microbiology DO - 10.15414/jmbfs.3807 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/3807 SP - e3807 AB - <p>Immunoglobulin G (IgG) are the essential tools in diagnostic kits. The whole-cell is still considered to be used for animal‘s immunization to produce IgG. The goal of this study was to determine dominant protein on the Shiga toxin-producing <em>Escherichia coli</em> (STEC) O157:H7-induced typical antibody response also determined by MALDI-TOF/TOF mass spectrometry. The heating method was conducted at 71 °C and 83 °C for antigen preparation. The proteins were characterized using SDS-PAGE and evaluated by MALDI-TOF/TOF mass spectrometry. The present study reported that the dominant polyclonal antibody inducing protein was ompC <em>E. coli</em>. The evaluation of mass spectra by MALDI-TOF/TOF might help to identify the dominant polyclonal antibody inducing protein in future preparation of diagnostic kits.</p> ER -