TY - JOUR AU - Abdel-Aziz, Moh. PY - 2021/08/01 Y2 - 2024/03/29 TI - TWO NOVEL CLUSTERS OF NOCARDIOPSIS RECOVERED FROM HALOALKALINE ENVIRONMENT: POTENTIAL RESOURCE OF MULTI-ADVANTAGEOUS LIPASES JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 11 IS - 1 SE - Microbiology DO - 10.15414/jmbfs.3924 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/3924 SP - e3924 AB - <p>Wadi El Natrun district in the northwest of Cairo, Egypt, represents a highly stable unique ecology containing dual stress of salinity and alkalinity. Bioprospecting lipase producers in such an environment helps in finding enzymes of novel properties. Seven&nbsp;<em>Nocardiopsis</em>&nbsp;strains were recovered from Wadi El Natrun region, and their distribution within the phylogeny of the genus showed a particular pattern of two clusters descending from two evolutionary lineages. All strains produced highly alkaline lipase (optimum pH: 9- 11) in different amounts. The principal features of lipases from all strains, not only from the most potent one, were realized in a comparative profile to prospect more of their possible advantages in industrial biocatalysis. Most of the lipases were thermophilic; the optimum temperature was 50-55°C in lipases from five isolates. Activation energy of all lipases varied considerably between 5.52 and 13.58 Kcal/mole. WN4L lipase exhibited the highest thermal stability (half-life time=99 min at 70°C). Interestingly, different lipases exhibited good stabilities in water-immiscible organic solvents which highlighted their suitability in the two-partitioning system applications. A general prospect about characteristic features of Wadi El Natrun lipases was realized; they share the multi-advantages of high alkalophilicity, tendency to be thermophilic, and readiness to work in acetone and water-immiscible solvents. Considering the extensive variation, each lipase appeared of a unique profile that enables to choose the most compatible one according to the specific circumstances of the interested industrial process. Moreover, a cocktail enzyme preparation of such lipases could be used as a multi-task lipase formula.</p> ER -