TY - JOUR AU - Elaoufi , Manal Maliha AU - Bouterfas , Karim AU - Djebbar , Ahmed Abdelhammid AU - Meziani, Samira AU - Rezki , Hanane AU - Kermas , Fatima AU - Benali , Mahmoud AU - Mokhtaria Yasmina, Boufadi PY - 2022/10/05 Y2 - 2024/03/29 TI - CHEMICAL COMPOSITION, ANTI-ULCER AND ANTI-INFLAMMATORY EFFECTS OF CAROB PODS (CERATONIA SILIQUA L.) POLYPHENOLS FROM AIN TEMOUCHENT JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 12 IS - 2 SE - Food Sciences DO - 10.55251/jmbfs.5427 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/5427 SP - AB - <p>This research was aimed to evaluate the chemical composition by HPLC/UV, anti-inflammatory effect <em>in vitro</em> (hyaluronidase assay) and anti-ulcer activity <em>in vivo</em> against gastric ulcer. This anti-ulcer activity was explored <em>in vivo</em> on Wistar rats exposed to a gastric ulcer provoked with oral administration of HCl /ethanol (150 mM /60%) for one day after five days of oral treatment with 250 mg/kg of ethanolic extract of carob pods (EECp), to restore stomach ulceration. Several compounds of carob have been identified by HPLC/UV. This identification reveals the existence of phenolic compounds; flavonoids (chrysin, galangin, pinocembrin, quercetin, genistein, kaempferol, etc.) and phenolic acids (rosmarinic acid, caffeic acids, ferulic acid, etc.). Our extract showed strong inhibitory activity of hyaluronidase with (IC<sub>50</sub> = 20.7 mg / ml). The results obtained indicated that administration of HCl /ethanol alone produced stomach ulceration in rats with very extensive and bleeding gastric lesions with 44.09% of percentage lesion and 362.26 mm<sup>2</sup> of lesions area in the stomach mucosa. However, pretreatement of rats with EECp revealed a spectacular inhibition of gastric mucosal damage (7.46%), a significant reduction in malondialdehyde (4.86 nmol/mL) and an increase in enzymes of oxidative status (superoxide dismutase, catalase and glutathione peroxidase). The present study results can attribute to carob an antioxidant, anti-inflammatory and anti-ulcer effects.</p> ER -