TY - JOUR AU - Aydın, Hanife Banu AU - Korkmaz, Serol AU - Omurtag Korkmaz, Burcu Irem PY - 2023/05/10 Y2 - 2024/03/28 TI - ANTIBACTERIAL AND ANTIVIRAL ACTIVITIES OF STINGING NETTLE (URTICA DIOICA L.) LEAF EXTRACT ON NOROVIRUS AND CAMPYLOBACTER JEJUNI AS FOODBORNE PATHOGENS JF - Journal of microbiology, biotechnology and food sciences JA - J microb biotech food sci VL - 13 IS - 1 SE - Food Sciences DO - 10.55251/jmbfs.9768 UR - https://office2.jmbfs.org/index.php/JMBFS/article/view/9768 SP - e9768 AB - <p>Research background. The cytotoxicity on various vital cell lines and the activity on foodborne pathogens (murine norovirus 1 as a norovirus surrogate and <em>Campylobacter jejuni</em>) of methanolic <em>Urtica dioica</em> L. leaf extract (UDE) were studied.</p><p>Experimental approach. The cytotoxic concentration of 50% (CC<sub>50</sub>) was measured by the linearity between UDE concentrations and cell viability. Antibacterial effects on <em>C.  jejuni</em> were analyzed by the broth microdilution method with a spectrophotometer. The virucidal and antiviral activities of UDE were determined by the virus titration method on the host cell infectivity and expressed as the tissue cell infective dose of 50% using the method of Spearman–Karber.</p><p>Results and conclusions. The CC<sub>50</sub> of UDE was determined on macrophage as the virus host cell. MIC and MBC of UDE were determined as 5 mg/ml and 20 mg/ml for both <em>C. jejuni</em> isolated from poultry meat and the standard strain. UDE inhibited MNV-1 on three pathways of host cell infectivity at approximately the same 50% inhibitory concentration (1.45-1.87 mg/ml). In conclusion, the present study tried to explain in detail the dose-dependent activity of <em>Urtica dioica</em> L. leaf extract on two important foodborne pathogens causing outbreaks worldwide. The results showed that it might be a safe and alternative food additive and supplement candidate at safe concentrations.</p> ER -