BIOTECHNOLOGICAL METHODS OF POTATO (SOLANUM TUBEROSUM L.) REPRODUCTION IN IN VITRO CULTURE USING ELEMENTS OF CHEMOTHERAPY
Keywords:potato, nutrient medium, chemotherapy, meristematic tissue culture, regeneration, morphogenesis in vitro
The elements of chemotherapy for the recovery of potato plants in vitro were selected. The influence of various modifications of the nutrient medium on changes in morphological parameters was investigated. The process of reproduction of potato plants of different varieties was studied. It was experimentally proved that in vitro potato recovery with elements of chemotherapy ensures the reproduction of the source material on a virus-free basis by inhibiting the activity of viruses and bacteria with virus inhibitory and antibacterial drugs. The absence of viruses in the obtained material is confirmed by the results of enzyme-linked immunosorbent assay (ELISA) diagnostics.
Studies have shown that the use of Noviryn is the most effective element of chemotherapy for Serpanok and Skarbnytsia varieties. Its action contributed to an increase in plant height and the number of leaves per shoot, and increased the multiplication factor to 4.5 and 3.9, respectively. The use of Noviryn in combination with Dekasan is the best option for improving Mezhyrichka 11 variety and leads to the improvement of certain morphological parameters (+4.5 cm of stem length, +3.5 of leaves) and the multiplication coefficient (+1.0). The use of Noviryn in combination with Salicylic Acid had a positive effect on changes in the morphological parameters of all potato varieties under study. It should be noted that during the growing season in the field, the first field generation of in vitro plants was resistant to secondary infection with viral diseases.
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Copyright (c) 2023 Iryna Sokolovska, Kateryna Vasylkovska, Mykola Mostipan, Olha Andriienko, Yevgeniia Shcherbyna
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