INOCULATION OF MULTI-TRAIT PLANT GROWTHPROMOTING RHIZOBACTERIA FOR WHEAT GROWTH PROMOTION IN POTHOUSE CONDITIONS

Abstract

Plant growth-promoting rhizobacteria (PGPR) are commonly used as inoculants to improve the growth and yield of wheat crops. Thus, the present study focused on the   isolating and screening of effective isolates with multi-traits PGPR activities related to plant growth and development. Bacterial strains RA4, RA11 and RA26 were showing plant growth promoting activities like potassium solubilization, phosphate solubilization, indole acetic acid production (IAA), hydrogen cyanide production (HCN), ammonium production, zinc solubilization, anti-fungal activity. The antibiotic pattern showed that bacterial isolates RA11 and RA26 were resistant against azithromycin, ampicillin and gentamicin. Identification of multi trait PGPR was carried out by 16s rRNA sequencing. Bacterial strains RA4, RA11 and RA26 were showing 99.93 %, 100 % and 99.97 % similarity and identified as Pseudomonas paraeruginosa, Bacillus cereus and Pseudomonas aeruginosa respectively. A pot house experiment was conducted in order to check the efficacy of multi-trait PGPR as inoculants for wheat growth. In our study, bacterial strain Bacillus cereus (RA11) was showing maximum plant growth promotion in the presence of the RDK of NPK in pot house conditions. Generally, results of this study revealed that use of bio fertilizers had an effective and significant role in the growth of plants.