ANTILISTERIAL ACTIVITY OF CBD FOR THE PREVENTION OF  LISTERIA MONOCYTOGENES  IN DAIRY PRODUCTS

Kazi Tahsin; David Watson; William Xu; David Heinrichs; Amin Rizkalla; Paul Charpentier

doi:10.55251/jmbfs.10659

Authors

Kazi Tahsin Western University London, Ontario, Canada, N6A 5B9 https://orcid.org/0000-0001-7939-5962
David Watson Department of Microbiology and Immunology, Western University, 1151 Richmond St, London, ON N6A 3K7, CA https://orcid.org/0000-0001-6755-7020
William Xu Department of Chemical & Biochemical Engineering, Western University, 1151 Richmond St, London, ON N6A 3K7 https://orcid.org/0000-0002-3460-8474
David Heinrichs Department of Microbiology and Immunology, Western University, 1151 Richmond St, London, ON N6A 3K7, CA https://orcid.org/0000-0002-7217-2456
Amin Rizkalla Department of Chemical & Biochemical Engineering, Western University, 1151 Richmond St, London, ON N6A 3K7 https://orcid.org/0000-0003-0548-3817
Paul Charpentier Department of Chemical & Biochemical Engineering, Western University, 1151 Richmond St, London, ON N6A 3K7

DOI:

https://doi.org/10.55251/jmbfs.10659

Keywords:

CBD, Listeria Monocytogenes, antimicrobial studies, dairy produce

Abstract

Research background. Herbal antimicrobials exist in plants and their derived compounds. One such compound is cannabidiol (CBD) extracted from the plant, Cannabis Sativa, also referred to as hemp or marijuana, has drawn interest for its alleged antibacterial and antioxidant abilities. With the increasing problem of antibiotic resistance and the desire to reduce antibiotics in the food industry, researchers are exploring the potential of cannabinoids as an alternative antimicrobial agent. The use of cannabidiol (CBD) in food and beverage products is a growing trend, it is also important for manufacturers to approach this new area with more studies. Milk is a key component in the production of most dairy produce. The products made from milk usually undergo various processes such as pasteurization, fermentation, curdling, and aging to create the final product. This study aims to examine the potential of CBD isolate as a antimicrobial agent which can be used in dairy products to reduce microbial growth and extend their shelf life.

Experimental approach. This paper investigated the antilmicrobial properties of CBD against L. monocytogenes in milk as the key solvent to perform studies. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of CBD in TSB was carried out at 37 °C in 24 h. CBD’s antilisterial activity was found for whole milk and skim milk at 4°C for 3 days by analyzing their respective growth and kill curves.

Results and conclusions Both the MIC and MBC for L. monocytogenes was found to be 2 µg/mL measured by dilution series and plating, respectively. CBD significantly slowed the growth of listeria populations in milk but its effectiveness of CBD was dependant on the fat content of milk. In this paper, we have discussed the potential for using and improving CBD as a preservative to combat L. monocytogenes in dairy products that use milk as a primary ingredient.