EVALUATION OF THE ANTIULCER AND ANTIDIARRHEAL POTENTIAL OF LACTIC ACID BACTERIA ISOLATED FROM YOGURT IN ULCER AND DIARRHEA INDUCED IN WISTAR RATS

David Adeiza Zakari; Precious Adejoh Idakwoji; Martins Abubala Okpanachi; Ebere Adaeze Onyebueke; Abraham Joseph Oyiguh; Yabefa Jeremiah Akpodoitei; Chukwuma  J. Taiwo Onwuatuegwu; Humphrey Afam Nwobodo; Kadiri Dickson Adejoh; Sunday Olusegun Abiodun; Onuh Kenneth Ikwulono; Patience Adejo Omebije; Idris Tsobaza Egbeja; Idris Mustapha Omenesa; Godwin Amoka Audu; Abdulbasit Anoze Aliyu; Abdulrahman Itopa Suleiman; Okeme Okwumonu Patrick

doi:10.55251/jmbfs.12330

Authors

David Adeiza Zakari Prince Abubakar Audu university PMB 1008, Anyigba, kogi atate https://orcid.org/0000-0002-7111-6065
Precious Adejoh Idakwoji prince abubakar audu university
Martins Abubala Okpanachi prince abubakar audu university
Ebere Adaeze Onyebueke university of nigeria, nsukka, Enugu
Abraham Joseph Oyiguh prince abubakar audu university
Yabefa Jeremiah Akpodoitei prince abubakar audu university
Chukwuma J. Taiwo Onwuatuegwu Tansian university, Umunya, Anambra State, Nigeria
Humphrey Afam Nwobodo Enugu State University of Science and Technology, Enugu State, Nigeria
Kadiri Dickson Adejoh Federal Ministry of Water Resources and Sanitation, Abuja, Nigeria.
Sunday Olusegun Abiodun Federal University of Agriculture, Abeokuta, Ogun state
Onuh Kenneth Ikwulono Federal University of Lafia, Nigeria
Patience Adejo Omebije prince abubakar audu university
Idris Tsobaza Egbeja prince abubakar audu university
Idris Mustapha Omenesa prince abubakar audu university
Godwin Amoka Audu prince abubakar audu university
Abdulbasit Anoze Aliyu prince abubakar audu university
Abdulrahman Itopa Suleiman School of Applied Sciences, Department of Science Laboratory Technology, Kogi State Polytechnic,P.M.B 1101, Lokoja,Nigeria. Directorate of Research and Innovation, Kogi State Polytechnic,P.M.B 1101, Lokoja,Nigeria
Okeme Okwumonu Patrick prince abubakar audu university

DOI:

https://doi.org/10.55251/jmbfs.12330

Keywords:

lactic acid bacteria, yogurt, antiulcer, antidiarreal, wistar rats, probiotics

Abstract

This study investigates the antiulcer and antidiarrheal potential of lactic acid bacteria (LAB) isolated from commercially available yogurt in Wistar rats. Three LAB strains, Lactobacillus paracasei (Y1), Lactobacillus rhamnosus (Y2), and Streptococcus thermophilus (Y3), were identified through biochemical tests and 16S rRNA sequencing. For the antiulcer study, rats were pre-treated with LAB strains for seven days before inducing gastric ulcers with indomethacin. Ulcer index and gastric juice parameters were assessed. For the antidiarrheal study, diarrhea was induced using castor oil, and the effects of LAB pre-treatment on fecal output, consistency, and intestinal transit time were evaluated. GC-MS analysis identified bioactive compounds that may contribute to the observed therapeutic effects. Results showed that L. paracasei (Y1) significantly reduced ulcer index by 65% (p<0.05) and increased gastric mucus production by 40% compared to the control group. L. rhamnosus (Y2) reduced diarrhea severity by 50% (p<0.05) and normalized intestinal transit time. S. thermophilus (Y3) showed moderate improvement in both ulcer and diarrhea parameters. Bioactive compounds such as lactic acid, acetic acid, butyric acid, and reuterin were identified, which may contribute to the protective effects. These findings suggest that LAB isolated from yogurt possess significant antiulcer and antidiarrheal properties, with L. paracasei and L. rhamnosus exhibiting the most promising effects.