EVALUATION OF POLYPHENOLIC CONTENT AND In VITRO ANTI COXSACKIE VIRUS, ANTI INFLAMMATORY, ANTIOXIDANT AND WOUND HEALING ACTIVITIES OF THE MUSHROOMS Ganoderma SP. AND Ganoderma applanatum EXTRACTS

Abstract

Ganoderma (immortality mushroom) is one of the key components of ancient Asian remedies used centuries ago for longevity and treatment of diseases. Inspired by its ethnopharmacological importance, Ganoderma is currently further studied in order to get maximum nutritional and pharmaceutical benefits. Hence, this study aimed to analyze polyphenolic composition and explore the in vitro bioactive capabilities of the ethyl acetate and n-butanol fractions of Ganoderma sp. and Ganoderma applanatum extracts. Ethyl acetate fractions were having higher phenolic and flavonoid contents, which influenced their antioxidant activity. The highest activity was recorded for G. applanatum ethyl acetate fraction. On the contrary, n-butanol fraction of G. applanatum exerted the highest nitric acid inhibition as an indication for its anti-inflammatory activity. On the other hand, studying effect of prepared Ganoderma fractions on coxsackie virus B3 (CVB3), responsible for viral myocarditis, revealed that during virucidal assay, Ganoderma sp. ethyl acetate fraction showed the highest antiviral activity against virus infection with reduction in virus titers (R) equal to 3.0 log₁₀ TCID₅₀, while during pre-treatment assay, the highest antiviral activities were recorded for G. applanatum ethyl acetate fractions with R=3.0 log₁₀ TCID₅₀. During post-treatment assay, the highest antiviral activity was achieved by G. applanatum n-butanol fraction with R=3.0 log₁₀ TCID₅₀. Concerning wound healing potentials of prepared Ganoderma fractions, investigating the migration of fibroblast cells as an indication for wound healing ability of fractions revealed that G. applanatum n-butanol fraction had the highest ability among all tested fractions. Further studies are encouraged.