EVALUATION OF THE DOMINANT PROTEIN FROM WHOLE-CELL HEATING METHOD USING MALDI-TOF/TOF MASS SPECTROMETRY

Authors

  • Siti Gusti Ningrum Universitas Wijaya Kusuma Surabaya
  • I Wayan Teguh Wibawan

DOI:

https://doi.org/10.15414/jmbfs.3807

Keywords:

STEC O157:H7, MALDI-TOF/TOF, SDS-PAGE, ompC

Abstract

Immunoglobulin G (IgG) are the essential tools in diagnostic kits. The whole-cell is still considered to be used for animal‘s immunization to produce IgG. The goal of this study was to determine dominant protein on the Shiga toxin-producing Escherichia coli (STEC) O157:H7-induced typical antibody response also determined by MALDI-TOF/TOF mass spectrometry. The heating method was conducted at 71 °C and 83 °C for antigen preparation. The proteins were characterized using SDS-PAGE and evaluated by MALDI-TOF/TOF mass spectrometry. The present study reported that the dominant polyclonal antibody inducing protein was ompC E. coli. The evaluation of mass spectra by MALDI-TOF/TOF might help to identify the dominant polyclonal antibody inducing protein in future preparation of diagnostic kits.

Downloads

Published

2021-06-01

How to Cite

Gusti Ningrum, S., & Wibawan, I. W. T. . (2021). EVALUATION OF THE DOMINANT PROTEIN FROM WHOLE-CELL HEATING METHOD USING MALDI-TOF/TOF MASS SPECTROMETRY. Journal of Microbiology, Biotechnology and Food Sciences, 10(6), e3807. https://doi.org/10.15414/jmbfs.3807

Issue

Section

Microbiology
Received 2020-10-08
Accepted 2021-01-20
Online Published 2021-06-01