CHARACTERIZATION OF A BOSEA SP. STRAIN SF5 (MTCC 10045) ISOLATED FROM COMPOST SOIL CAPABLE OF PRODUCING CELLULASE

Authors

  • Sangrila Sadhu
  • Pradipta Saha
  • Shanmugam Mayilraj
  • Tushar Kanti Maiti

Keywords:

CMCase, Avicelase, FPase, β-glucosidase, Bosea sp.

Abstract

A cellulase producing bacterium, designated SF5 was isolated from compost soil. The strain was identified as Bosea sp. based on 16S rRNA gene sequence analysis and phenotypic characters including detail carbon sources utilization pattern. The effect of various carbohydrates such as Carboxy Methyl Cellulose (CMC) avicel, starch, maltose, sucrose, glucose, fructose and lactose (as carbon source) on cellulase production revealed that 0.75% CMC (with 8 days incubation) was optimum. Among the various nitrogen sources, 0.15% NH4Cl gave optimal production of cellulase. The optimal conditions for the production of cellulase by strain SF5 were determined to be at 37 ºC temperature and at pH 7.0. The strain is also capable of producing xylanase and may have biotechnological potential.

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Published

2012-10-01

How to Cite

Sadhu, S., Saha, P., Mayilraj, S., & Kanti Maiti, T. (2012). CHARACTERIZATION OF A BOSEA SP. STRAIN SF5 (MTCC 10045) ISOLATED FROM COMPOST SOIL CAPABLE OF PRODUCING CELLULASE. Journal of Microbiology, Biotechnology and Food Sciences, 2(2), 576–591. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7166

Issue

Vol. 2 No. 2 (2012): October - November

Section

Microbiology

License

Copyright (c) 2012 Sangrila Sadhu, Pradipta Saha, Shanmugam Mayilraj, Tushar Kanti Maiti

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Journal of Microbiology, Biotechnology and Food Sciences  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).