DYNAMICS OF SOME BIOCHEMICAL PARAMETERS IN THE BLOOD SERUM OF MOUFFLONS IN THE PERIOD OF SYNCHRONIZATION TREATMENT AND ON THE DAYS OF OBSERVATION AFTER INSEMINATION

Abstract

The 10 moufflons of 3-7 years of age were included in the experiment. Their weight range was from 32 to 40 kg. The animals were kept in a game preserve and their food consisted of pasture and water were unlimited. Heat is conducted in the month of November. Blood was collected from the jugular vein before synchronization (day 0) and on days 5, 8, and 12 during treatment with tampon Chronogestcrvag. spong. a.u.v. (Cronolone 20 mg / tampon). To induce oestrus in moufflons eCG was used during oestrus and they were fertilized. Sampling continued in the days and on days 5, 12, 18 and 33 after oestrus. Sodium concentration and K were determined in serum by absorption spectrometry method and tools AtomspecFY-RangHigler. The concentration of Ca, P, total lipids and cholesterol were determined using BIO-Lachematests (Lachema, Brno). A statistically significant decrease was observed at the peak of oestrus (p <0.01) and also at the end of the trial (p <0.001). K value decreased, were statistically significant (p <0.01) in the days and after oestrus in the days following oestrus 5, 12, 18 and 33 (p <0.001). Concentration of Ca tended fall, were statistically significant (p <0.05, p <0.01, p <0.001, p <0.001) the investigated days. Significant decrease (p <0.05), the concentration of P is from 5 day after oestrus. Mean levels of total lipids significantly diminished after oestrus (p <0.05) and were in the reference range. Starting 5 days after oestrus and at end of the experiment the concentration in the range of 1.59 ± 0.47 to 1.81 ± 0.49 gl-1.