SUPPLEMENTATION OF FRUIT PROCESSING WASTE FOR ENDOXYLANASE PRODUCTION BY TRICHODERMA KOENINGI ISOLATE AND ITS OPTIMIZATION USING CENTRAL COMPOSITE DESIGN: APPLICATION OF PRODUCED ENDOXYLANASE IN MANGO JUICE CLARIFICATION

Abstract

The aim of this study was to optimize the supplementation of custard apple, pomegranate peel along with KH2PO4, (NH4)2SO4 and MgSO4 for maximum endoxylanase production by Trichoderma koeningi isolate on pretreated corn cobs using CCD. Predicted endoxylanase yield was highest (3242.43 U/g) with the actual yield of (3107.58U/g) from 2% NaOH pretreated corn cobs. The model predicted R2 value of 0.9147% indicating that it was appropriate to predict the levels of variables to achieve maximum endoxylanase yield. The produced endoxylanase clarified mango juice of Baneshan and Totapuri varieties at 40°C and 56 h. The endoxylanase treatment enhanced the clarity of Baneshan juice by 51.7% with a release of 121.1 mg/ml of reducing sugars at an optimized enzyme dose of 9.6 U that led to maximum flow rate of 8 ml/min. In the case of Totapuri juice, there was 43.4% increase in clarity and release of 102.6 mg/ml reducing sugars at an optimized enzyme dose of 12.8 U that lead to a maximum juice flow rate of 6 ml/min. The HPLC analysis showed that the hydrolysed products of mango juice were xylose and xylo-oligosacharides. An optimized xylanase production was achieved from low cost agro residues, which results in the reduction of enzyme cost.