In VIVO EFFECT of AMPICILLIN, ENROFLOXACIN, COLISTIN, and SULFONAMIDES on SPERM PARAMETERS in BREEDING ROOSTERS

Abstract

The present study aimed to investigate the effect of commonly prescribed antibiotics on sperm parameters in breeding roosters. Twenty adult subjects were divided on the basis of their response to the dorso-abdominal massage into four experimental groups (G1: 30 mg/kg of ampicillin, G2: 10 mg/kg of enrofloxacin, G3: 2.5 mg/kg of colistin, and G4: 140 mg/kg of a sulfonamides association (sulphaquinoxaline sodium 150 mg, sulphamethazine sodium 70 mg, sulphadiazine sodium 70 mg). The measurements were performed at (T0) (before treatment), (T3 and T9) (representing 3 and 9 days of treatment) by Computer Aided Sperm Analysis (CASA). All antibiotics did not affect ejaculate color, pH, seminal fluid viscosity, and agglutination. Ampicillin increased slightly sperm volume at T3 (0.31±0.19) with no significant change at T9 (0.30±0.15). No significant effect was observed at T3 on sperm count (224.05±41.9), viability (94±2.08), and total motility (TM) (57%). However, progressive motility (PM) deceased slightly on T3 (36.92%). Enrofloxacin and colistin induced a significant decrease in sperm volume (0.40±0.10, 0.16±0.08), count (2.13±0.27, 0.65±0.33), viability (81.33±5.45, 37.33±18.67), TM (54.95%, 46.91%), and PM (31.24%, 23%) on T3. On T9 some parameters were improved to be closer to those observed at T0. Ampicillin, colistin, and enrofloxacin decreased significantly all (CASA) kinematic parameters. The highest impact was observed on the third day of treatment (T3). Sulfonamides decreased sperm volume and gametes concentration but enhanced significantly sperm viability, total motility TM, progressive motility PM, and all kinematic parameters.