FLAVONOID AND ANTIOXIDANT ACTIVITY ANALYSIS OF ANTHOCYANIN BLACK RICE BRAN EXTRACT (ABRIBE) CV CEMPO IRENG ORIGIN FROM INDONESIA

Abstract

Black rice is an indigenous food in Indonesia that is rich in anthocyanins, a group of plant pigments that are also found in berries. Despite the similarity in anthocyanin content, the high cost and limited availability of berries have restricted their use for extraction. Therefore, this study aimed to determine the total anthocyanin, flavonoid content, and antioxidant activity of black rice bran. To achieve this, the extraction was conducted with maceration using ethanol-citric acid and freeze-drying techniques. The process involved several stages, such as making black rice bran powder, maceration, homogenization, filtration, evaporation, and freeze-drying. The UV-Vis spectrophotometer was used to determine the total anthocyanin and flavonoid content, as well as antioxidant activity. The result showed a total anthocyanin content and the total flavonoid content of 2.48 ± 0.17 mg/gram and 3.76 ± 0.000 mg QE/g freeze-dried extract, respectively. Furthermore, the antioxidant activity of black rice bran CV Cempo Ireng extract and ascorbic acid as a standard was measured in terms of half maximal inhibitory concentration (IC₅₀) in the form of percentage inhibition (%I), with values of 112.42 ± 2.368 and 7.18 ± 0.042 μg/mL, respectively.