BACILLUS SUBTILIS EMEA 22, POTENT MANGROVE RHIZOSPHERE AMYLASE PRODUCER: ISOLATION AND CHARACTERIZATION

Abstract

Amylases are one of the principal biocatalysts with significant applications in the fields of food, fermentation, textile, paper, detergent, and pharmaceutical industries. The present study focused on Bacillus subtilis EMEA 22, a halo-tolerant inhabitant of the soil of mangrove ecosystems capable of producing a greater amount of amylase. The whole isolates from different soil samples were further screened for amylase activity by introducing them into the starch agar. Furthermore, the amylase assay was also inspected. The bacteria with potential amylase activity were identified by biochemical and molecular characterization. Partial purification of the enzyme was carried out, which was further characterized by holding it at different temperatures, pH, and in the presence of metal ions. The outcome of which revealed that the isolated enzyme has the potential to maintain its maximal activity at pH ranges 7-9. The enzyme exhibited a high rise in activity in the presence of CaCl2 at 1 mM concentration, moreover, the study demonstrated the enzyme's textile starch removal capacity.