IN VITRO EVALUATION OF THE BIOLOGICAL ACTIVITY POTENTIAL OF ALOE VERA GEL: ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECTS IN HEPG2 AND L929

Abstract

Background: The place of natural and herbal products in alternative therapy is very important. A.vera is also an important plant that can be used in alternative therapy. Our study aimed to determine the antioxidant activity and cytotoxic effects of Aloe vera gel on HepG2 and L929 cell lines. Methods: The effects of A.vera on HepG2 and L929 were evaluated via the MTT analysis. The total antioxidant status and DPPH methods were used to determine the antioxidant capacity. Results: In our study, the mean cytotoxic effect on HepG2 of A.vera was determined to be 60.47 mg/mL at 24 hours and 44.64 mg/mL at 48 hours. In a healthy L929 fibroblast cell line, the best stimulating effect of A. vera was in 48 hours, and the EC50 value was calculated as 21.98±18.87 µg/ml. TAS was determined to be 0.2 mmol/L in A. vera. Free radical scavenging activity was determined at 21.58 mg/mL Conclusions: According to the obtained data, it was determined that A. vera gel has a concentration- and time-dependent cytotoxic effect on HepG2, while it has a proliferative effect on healthy cells. The fact that A. vera gel has antioxidant capacity, together with its positive effects on cells, shows that it can be an important support in treatments.