ENTEROBACTER CLOACAE LIPOPOLYSACCHARIDE EXPORT SYSTEM PROTEIN (LPTC) GENE EXPRESSION VARIATION VIA EXPOSE TO BIOSYNTHESIZED ZINC OXIDE NANOPARTICLES

Abstract

Synthetic nanoparticles (NPs) have become more widely used in the disinfection and health sectors owing to their ability to effectively penetrate biological systems. Recent research has demonstrated the ability of microbial proteins and enzymes to function as reducing agents throughout the NP-production process, providing a different option to chemical and physical techniques. This method not only saves money and is effective, but also has the least possible environmental impact. This work used Lactobacillus spp., as the reducing and capping agent to create zinc oxide nanoparticles, (ZnO NPs). Numerous analytical techniques were employed to examine the generated ZnO NPs; these techniques include ultraviolet–visible spectroscopy (UV–vis), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). As an opportunistic pathogenic bacterium, Enterobacter cloaca possesses several virulence characteristics that enable it to infiltrate target tissues. However, bacteria can become resistant to antibiotics, which in turn makes treating bacterial infections becomes more difficult. With the development of science at the biotechnology level, using zinc oxide ZnO NPs on isolates of Enterobacter cloacae, we conducted research in the promising field of nanotechnology. The impact of three different concentrations of ZnO NPs on the gene expression of Enterobacter cloaca lipopolysaccharide transport (Lpt) proteins in isolates obtained from some patients suffering of various infections was investigated. Bacteria with the highest multidrug resistance were selected for further analysis. Expression of LptC gene was reduced significantly at concentration of 125 mg/mL (P < 0.01) for all the isolate that treated with ZnO NPs.