IN SILICO ANALYSIS OF THE FUNCTIONAL AND SAFETY ASSESSMENTS OF LACTIPLANTIBACILLUS PLANTARUM BBS13 ISOLATED FROM LAO TRADITIONAL FERMENTED BAMBOO SHOOT (NOR MAI SOM) BASED ON WHOLE GENOME SEQUENCING

Abstract

In this study, the genome of strain BBS13 was sequenced using the Illumina HiSeq2500 platform to determine its classification, annotate its main features, and to evaluate its safety characteristics. Genome analysis revealed an average nucleotide identity (ANI) of 99.05% with Lactiplantibacillus plantarum. The circular chromosome of BBS13 comprises 3,238,769 bp with a GC content of 44.45%, and a total of 3,054 protein-coding sequences (CDSs) were assigned putative functional roles.

The functional annotation analysis by Rapid Annotation using Subsystems Technology (RAST) integrated with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that the strain BBS13 possesses genes encoding L-acetate dehydrogenase (L-LDH; EC 1.1.1.27) and D-lactate dehydrogenase (D-LDH; EC 1.1.1.28), which are responsible for lactic acid production, as well as linamarase or β-glucosidases (EC 3.2.1.21). Interestingly, through the safety assessments via WGS, no virulence factors, biogenic amines, or antimicrobial resistance genes was found in this strain. Our previous studies showed probiotic properties of this strain such as tolerance to the simulated artificial gastrointestinal tract, bacterial adhesion, antibacterial activity, and antioxidant function. The findings presented herein greatly boosted the level of information available on BBS13 in support of its potential application in food products for health promotion.