IN VITRO EVALUATION OF ANTIMICROBIAL, ANTI-INFLAMMATORY AND CYTOTOXIC PROPERTY AGAINST HEPG2 CELL LINE OF ESSENTIAL OIL EXTRACTED FROM THYMUS VULGARIS LEAVES

Abstract

Thymus vulgaris, also known as thyme, is being used as a versatile herb with both culinary and medicinal applications. It also incorporated into skincare and haircare products due to its antimicrobial and soothing properties. Recent studies highlighted its potential therapeutic benefits. Thyme essential oil was extracted via the hydro-distillation method. Gas chromatography (GC-MS) analysis revealed bioactive substances found in the essential oil. Additionally, leaf extracts were evaluated for various biological functions, including antioxidant, anti-inflammatory, and antimicrobial properties. Total phenol content was examined through the Folin-Ciocalteau's method, and Antioxidant activity was measured through DPPH assay. Antimicrobial activity against various bacterial and fungal pathogens was tested using the disc diffusion method. Furthermore, cytotoxicity activity was evaluated by assessing the cell viability of the HEPG2 cell line. These findings underscored the potential of thyme as a provider of therapeutically useful bioactive properties.