STATISTICAL OPTIMIZATION OF ALKALINE LIPASE PRODUCTION BY EXTREME HALOPHILIC ARCHEAN NATRIALBA ASIATICA

Authors

  • Maryam Pirghorbani Department of Microbiology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
  • Mehdi Ebrahimi Department of Biochemistry and Biophysics, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran

DOI:

https://doi.org/10.15414/jmbfs.1164

Keywords:

Lipase, Natrialba asiatica, Plackett–Burman Design, Response Surface Methodology, Extreme halophilic archaea

Abstract

In this study, extreme halophilic archean Natrialba asiatica was utilized as a new source for lipase production. Lipases from halophilic archaea are appealing for utilization in assorted industrial and biotechnological applications. The optimum temperature and pH of N. asiatica lipase in the crude mixture were 50 °C and 10, respectively. The growth conditions influencing lipase production were determined using a two-level fractional factorial Plackett–Burman design. Among the 9 factors screened, MgCl2 concentration, temperature, and shaking were found to be effective. The optimum levels of these factors for the production process were determined by employing the central composite design of response surface methodology. The 27 g L-1 of MgCl2, 50 °C, and 133 rpm were determined as optimized conditions for lipase production. The enzyme activity increased from 3.39 to 6.1 U mL-1 using predicted optimum levels. These findings help understanding factors affecting the production of lipase by halo-archean N. asiatica. Moreover, using the optimized level of temperature, shaking, and MgCl2, it is possible to increase the production of valuable alkaline lipase by N. asiatica.

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Published

2021-05-11

How to Cite

Pirghorbani, M., & Ebrahimi, M. (2021). STATISTICAL OPTIMIZATION OF ALKALINE LIPASE PRODUCTION BY EXTREME HALOPHILIC ARCHEAN NATRIALBA ASIATICA. Journal of Microbiology, Biotechnology and Food Sciences, e1164. https://doi.org/10.15414/jmbfs.1164

Issue

Section

Biotechnology
Received 2019-02-04
Accepted 2021-03-04
Online Published 2021-05-11