STATISTICAL OPTIMIZATION OF ALKALINE LIPASE PRODUCTION BY EXTREME HALOPHILIC ARCHEAN NATRIALBA ASIATICA

Abstract

In this study, extreme halophilic archean Natrialba asiatica was utilized as a new source for lipase production. Lipases from halophilic archaea are appealing for utilization in assorted industrial and biotechnological applications. The optimum temperature and pH of N. asiatica lipase in the crude mixture were 50 °C and 10, respectively. The growth conditions influencing lipase production were determined using a two-level fractional factorial Plackett–Burman design. Among the 9 factors screened, MgCl₂ concentration, temperature, and shaking were found to be effective. The optimum levels of these factors for the production process were determined by employing the central composite design of response surface methodology. The 27 g L^-1 of MgCl₂, 50 °C, and 133 rpm were determined as optimized conditions for lipase production. The enzyme activity increased from 3.39 to 6.1 U mL^-1 using predicted optimum levels. These findings help understanding factors affecting the production of lipase by halo-archean N. asiatica. Moreover, using the optimized level of temperature, shaking, and MgCl2, it is possible to increase the production of valuable alkaline lipase by N. asiatica.