HETEROLOGOUS EXPRESSION OF A GENE ENCODING BIPHENYL 2,3-DIOXYGENASE FROM BURKHOLDERIA CEPACIA IN ESCHERICHIA COLI

Authors

  • Nguyen Hoang Loc Hue University
  • Duong Duc Hoang Sinh
  • Nguyen Hoang Tue
  • Nguyen Duc Huy
  • Tran Vu Ngoc Thi
  • Le Thi Ha Thanh

DOI:

https://doi.org/10.15414/jmbfs.2020.10.3.490-492

Keywords:

Biphenyl 2,3-dioxygenase, BphA gene, Burkholderia cepacia, heterologous expression

Abstract

Biphenyl 2,3-dioxygenase (BphA) is an enzyme that participates in initiation step of the pathway of biphenyl degradation in bacteria. Biphenyl is an aromatic hydrocarbon including two benzene rings that causes a serious environmental pollution. This report presents the preliminary results on the cloning and expression of BphA gene of B. cepacia strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam. The coding DNA sequences of BphA genes are 897 bp long and 99% identical. Recombinant BphA enzyme (33 kDa) has strongly expressed in E. coli M15 after induction of 0.5 mM IPTG for 4 h at 37ºC.

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Published

2020-12-01

How to Cite

Loc, N. H., Sinh, D. D. H., Tue, N. H., Huy, N. D., Ngoc Thi, T. V. ., & Ha Thanh, L. T. . (2020). HETEROLOGOUS EXPRESSION OF A GENE ENCODING BIPHENYL 2,3-DIOXYGENASE FROM BURKHOLDERIA CEPACIA IN ESCHERICHIA COLI. Journal of Microbiology, Biotechnology and Food Sciences, 10(3), 490–492. https://doi.org/10.15414/jmbfs.2020.10.3.490-492

Issue

Section

Biotechnology
Received 2019-05-09
Accepted 2020-08-12
Online Published 2020-12-01