A A NEW SIMPLE METHOD TO PRESERVE PHAGE - PRELIMINARY STUDY: PRELIMINARY STUDY

ANURADHA ATUL JAPE

doi:10.15414/jmbfs.1710

A A NEW SIMPLE METHOD TO PRESERVE PHAGE - PRELIMINARY STUDY

PRELIMINARY STUDY

Authors

ANURADHA ATUL JAPE BVDU, Y. M. COLLEGE OF ARTS SCIENCE AND COMMERCE,DEPARTMENT OF MICROBIOLOGY PUNE

DOI:

https://doi.org/10.15414/jmbfs.1710

Keywords:

phage preservation, alginate, encapsulation of host infected with phage (EHIP), normal refrigeration

Abstract

The host E. coli and its phage were isolated from sewage water. The phage infected host, E coli cells were encapsulated in alginate at specific time interval of co-cultivation, beads were stored at different temperatures. Beads were solublised to release phages and stability of released phages was evaluated by performing plaque assay at monthly interval. The number of plaque forming units increased with time of co-cultivation and by 45mins of phage-host co-cultivation, count raised from 10⁶to 10⁸pfu/ml. The infected host cells were entrapped in calcium alginate at a stage when multiple copies of mature phage are ready within host cell, and well before commencement of cell lysis.

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Published

2021-04-01

How to Cite

JAPE, A. A. (2021). A A NEW SIMPLE METHOD TO PRESERVE PHAGE - PRELIMINARY STUDY: PRELIMINARY STUDY. Journal of Microbiology, Biotechnology and Food Sciences, 10(5), e1710. https://doi.org/10.15414/jmbfs.1710

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Issue

Vol. 10 No. 5 (2021): April - May

Section

Microbiology

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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