The EVALUATION OF ACUTE TOXICITY EXPOSURE TO AQUEOUS LEAVES EXTRACT OF SYZYGIUM KANARENSE (TALBOT) RAIZADA

Abstract

Syzygium kanarense (Talbot) Raizada (Family: Myrtaceae), a rare plant tested for toxicity in order to explore therapeutic value. In the current study, the preliminary phytochemical screening, in vitro toxicity and acute toxicity of aqueous extract of Syzygium kanarense leaves (AESKL) were evaluated. Phytochemicals were screened and cytotoxicity was confirmed by MTT assay using NIH/3T3 cell line. For acute toxicity evaluation, female rats were divided into two equal groups; one group served as control while the other group received a single dose of AESKL at 2000mg/kg body weight (b.w) were treated and observed for 14 days. AESKL contains phenolics, tannins, terpenoids, saponins and steroids. The result showed the cytotoxicity value of inhibitory concentration (IC₅₀) = 491.56 µg/ml of AESKL. Acute toxicity of AESKL established the Lethal Dose (LD₅₀) more than 2000 mg/kg b.w and no mortality rate was observed. No changes in behavioural, b.w, relative organ weight, haematological, biochemical and histological were observed. Based on the results, it can be conclude that AESKL do not pose any risk when given orally to rats. To the best of our knowledge, this is the first report on evaluation of in vitro and in vivo toxicity of AESKL.