PHYSIOLOGICAL ALTERATIONS OF MITOCHONDRIA UNDER DIABETES CONDITION AND ITS CORRECTION BY POLYPHENOL GOSSITAN

Abstract

The state of lipid peroxidation (LPO), respiration and oxidative phosphorylation, mitochondrial permeability transition pore (mPTP) and antiradical activity of rat liver mitochondria in streptozotocin (STZ)-induced diabetes condition were studied, considered the ways of correction of detected membrane damages with the use of gossitan, isolated from cotton plant Gossypium hirsutum L. It was shown that the rate of respiration of liver mitochondria in states V₃ and V₄ increases during STZ-induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The findings suggest that the uncoupling of respiration and oxidative phosphorylation takes place during STZ-induced diabetes. It was shown that in the conditions of STZ-induced diabetes, the rate of swelling of rat liver mitochondria is higher than of the healthy ones; this means that mPTP of rat liver mitochondria is in the open state. Gossitan recovers mPTP to the normal condition, thereby removing the effect of STZ on mitochondria. Gossitan (per os dose is 10 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver mitochondria, probably due to its antioxidant properties.