PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile)

fatemeh shayesteh; Asmat  Ahmad; Gires  Usup

doi:10.15414/jmbfs.2513

PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile)

Authors

fatemeh shayesteh Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.
Asmat Ahmad
Gires Usup

DOI:

https://doi.org/10.15414/jmbfs.2513

Keywords:

antifungal bacteriocin, Marine bacteria, Candida albicans, Bacillus sp., purification

Abstract

This study was conducted to purify and characterize an anti- C. albicans bacteriocin produced by Bacillus sp. Sh10 isolated from marine carpet clam, Paphia textile. Extracellularly produced bacteriocin was purified by 80 % ammonium sulfate precipitation and size exclusive gel chromatography using superdex-200 column chromatographyThe purification steps resulted in 3.68-fold increase in specific activity and recovery of 20.66 % of total activity. Â The purity rate and molecular mass of 11 KDa of this compound were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Â Profile analysis of this bacteriocin by electrospray mass spectrometry (LC/MS/MS) showed 43% protein sequence coverage with a putative uncharacterized protein from Bacillus cereus strain B4264.

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Published

2021-06-01

How to Cite

shayesteh, fatemeh, Ahmad, A. ., & Usup, G. . (2021). PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile). Journal of Microbiology, Biotechnology and Food Sciences, 10(6), e2513. https://doi.org/10.15414/jmbfs.2513

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Issue

Vol. 10 No. 6 (2021): June - July

Section

Microbiology

License

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