PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile)

Authors

  • fatemeh shayesteh Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.
  • Asmat Ahmad
  • Gires Usup

DOI:

https://doi.org/10.15414/jmbfs.2513

Keywords:

antifungal bacteriocin, Marine bacteria, Candida albicans, Bacillus sp., purification

Abstract

This study was conducted to purify and characterize an anti- C. albicans bacteriocin produced by Bacillus sp. Sh10 isolated from marine carpet clam, Paphia textile. Extracellularly produced bacteriocin was purified by 80 % ammonium sulfate precipitation and size exclusive gel chromatography using superdex-200 column chromatographyThe purification steps resulted in 3.68-fold increase in specific activity and recovery of 20.66 % of total activity.  The purity rate and molecular mass of 11 KDa of this compound were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).  Profile analysis of this bacteriocin by electrospray mass spectrometry (LC/MS/MS) showed 43% protein sequence coverage with a putative uncharacterized protein from Bacillus cereus strain B4264.

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Published

2021-06-01

How to Cite

shayesteh, fatemeh, Ahmad, A. ., & Usup, G. . (2021). PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile). Journal of Microbiology, Biotechnology and Food Sciences, 10(6), e2513. https://doi.org/10.15414/jmbfs.2513

Issue

Section

Microbiology
Received 2020-01-04
Accepted 2020-10-27
Online Published 2021-06-01