CORRELATION STUDIES OF INDIGENOUS BIOFILM FORMING BACTERIA FOR RESISTANCE AGAINST SELECTED METALS, ANTIBIOTICS AND DYES DEGRADATION

Authors

  • Uruj Tahir Fatima Jinnah Women University
  • Shiza Nawaz
  • Umair Hassan Khan
  • Azra Yasmin

DOI:

https://doi.org/10.15414/jmbfs.2759

Keywords:

Biofilms, metal/antibiotic resistance, dye decolorization, correlation coefficient, phylogenetic identification

Abstract

This study determines correlation of biofilm-forming bacteria for selected metals, antibiotics and dyes. Total 12 strains having biofilm production potential were targeted in this study. Majority of them were Gram negative excluding MB377 and MB378, and facultative anaerobes. Isolates were mesophilic and exhibited growth capacity over a wide variety of pHs. Almost all isolates conferred resistance capacity against a wide range of multiple antibiotics having 0.0063 MAR for bacitracin, ampicillin, clindamycin, amoxicillin and sulphamethoxazole. Bacterial isolates displayed tolerance to diverse variety of metals i.e. cadmium, lead and iron 1000 mg L-1, copper 600-1000 mg L-1, chromium 700-1000 mg L-1 and nickel 200-600 mg L-1. Maximum heavy metal tolerance of 0.033 was observed for all metals excluding nickel. Correlation among metal tolerance and antibiotic resistance was extremely significant for chromium and bacitracin 0.862. Furthermore, moderate relationship (r= 0.682) between copper resistance and Basic Green 4 dye degradation was observed. Correlation coefficient for cadmium and Acid Red 2 dye was recorded as 0.512, while that for bacitracin and Basic Green 4 was noted as 0.665. These correlations proposed that resistance conferring genes might be present on same locus, genetic elements, chromosome or on a plasmid. 16S rRNA sequenced isolates were identified to be Klebsiella pneumoniae MB375, Staphylococcus sp. MB377, Klebsiella oxytoca MB381, Klebsiella pneumoniae MB394 and Klebsiella pneumoniae MB398. Potential of these biofilm forming strains to thrive under stressed environmental conditions proves them good entrants for being used in bioremoval of polluted sites after further studies on their metabolic pathways.

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Published

2021-05-07

How to Cite

Tahir, U., Nawaz, S., Khan, U. H., & Yasmin, A. . (2021). CORRELATION STUDIES OF INDIGENOUS BIOFILM FORMING BACTERIA FOR RESISTANCE AGAINST SELECTED METALS, ANTIBIOTICS AND DYES DEGRADATION. Journal of Microbiology, Biotechnology and Food Sciences, e2759. https://doi.org/10.15414/jmbfs.2759

Issue

Section

Microbiology
Received 2020-03-12
Accepted 2021-02-24
Online Published 2021-05-07