SUBTILISIN AN EMINENT MICROBIAL PRODUCT AS A POTENTIAL SKIN CARE REGULATOR OF MELANOGENESIS –A PARADIGM SYNCHRONIZED WITH IN VITRO / IN SILICO APPROACH

Authors

  • Arockiya Anita Margret Bishop Heber College
  • Irulandi Nivetha
  • Arockiya Avila Jerley
  • S Aishwarya

DOI:

https://doi.org/10.55251/jmbfs.2847

Keywords:

Melanogenesis, skin, Tyrosinase-related protein, Bacillus subtilis, Subtilisin, cosmetics, molecular docking

Abstract

Colour is considered as a foremost parameter for physical appearance and personal identification. The distinct coloration of skin, hair and eyes of humas are categorized based on the natural pigment melanin which is the final product of melanogenesis. Melanin establishes a primary protection to the skin by inducing photo protection. But the rapid mechanism of absorbing free radicals from the cytoplasm is defensive against UV light, thereby its enormous production   and accretion leads to perturbing skin ailments. During the process of melanogenesis the core enzyme tyrosinase is employed and moderated by a main transcription factor called microphthalmia associated transcription factor (MITF), which is enhanced by both tyrosinase-related 1 and 2 proteins (TRP-1 /TRP-2). Amending this physiological process is considered to be the foremost mechanism in improving skin fairness. Conversely, there are numerous cosmetics commercially available to depigment skin colour, the alarm of adverse effects and reoccurrence of hyperpigmentation prevails as a hitch. Synthetic skin products cause remedy with adverse effects and hence there is a high demand for novel skin colouring agent. This work lays a pedal stone in promoting the appliance of a naturally derived protein subtilisin secluded from soil isolates of Bacillus sp. in cosmetic industry as skin whitening aspect. The extraction of subtilisin was detected by biochemical and HPLC assays coordinated with anti-melanogenesis activity by in vitro studies. Further, synchronization of   molecular docking studies hoisted the protein as an effective ligand targeting TRP1 and TRP2 with energy minimization of -675569 KJ/Mol and -36957KJ/Mol.

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Published

2022-08-01

How to Cite

Margret, A. A., Nivetha, I., Jerley, A. A., & Aishwarya, S. (2022). SUBTILISIN AN EMINENT MICROBIAL PRODUCT AS A POTENTIAL SKIN CARE REGULATOR OF MELANOGENESIS –A PARADIGM SYNCHRONIZED WITH IN VITRO / IN SILICO APPROACH. Journal of Microbiology, Biotechnology and Food Sciences, 12(1), e2847. https://doi.org/10.55251/jmbfs.2847

Issue

Section

Biotechnology
Received 2020-03-29
Accepted 2020-11-23
Online Published 2022-08-01