VISIBLE/NEAR-INFRARED (VIS/NIR) SPECTROSCOPY TECHNIQUE TO DETECT GRAY MOLD DISEASE IN THE EARLY STAGES OF TOMATO FRUIT

VIS/NIR spectroscopy for detecting gray mold in tomato

Authors

  • Khadeja Najjar Department of Agricultural Biotechnology, College of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O.Box 7, Tulkarm, Palestine
  • Nawaf Abu-Khalaf Department of Agricultural Biotechnology, College of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O.Box 7, Tulkarm

DOI:

https://doi.org/10.15414/jmbfs.3108

Keywords:

gray mold;, visible/near-infrared (VIS/NIR) spectroscopy, early detection, multivariate data analysis (MVDA), principal component analysis (PCA)

Abstract

Fungal diseases are among the most common problems that affect tomato fruits, especially after harvesting. The early detection of these diseases may save 60% of the losses. Thus, this study aimed to detect the gray mold disease at an early stage on tomato fruits using the non-destructive visible/near-infrared (VIS/NIR) technique. Botrytis. cinerea fungus was isolated and identified using two specific primer sets (C729+/- and BC108+/BC563-). Three tomato varieties were used for this study (i.e. Harver, Izmer and Ekram), 30 samples from each variety, 20 samples were injected with the B. cinerea and 10 samples were left as control/healthy samples. Samples were examined using the VIS/NIR spectroscopy with a range of 550-1100 nm for three days. Thereafter, the data were analyzed using principal component analysis (PCA), where the results showed the ability of VIS/NIR to detect the infected samples on the second day of the injection, and before the symptoms appear on the samples. Two PCs explained 99% and 100% of VIS/NIR variance, respectively.

Author Biographies

Khadeja Najjar, Department of Agricultural Biotechnology, College of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O.Box 7, Tulkarm, Palestine

Department of Agricultural Biotechnology, College of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O.Box 7, Tulkarm, Palestine

Nawaf Abu-Khalaf, Department of Agricultural Biotechnology, College of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O.Box 7, Tulkarm

Department of Agricultural Biotechnology, College of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O.Box 7, Tulkarm, Palestine

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Published

2021-05-07

How to Cite

Najjar, K., & Abu-Khalaf, N. (2021). VISIBLE/NEAR-INFRARED (VIS/NIR) SPECTROSCOPY TECHNIQUE TO DETECT GRAY MOLD DISEASE IN THE EARLY STAGES OF TOMATO FRUIT: VIS/NIR spectroscopy for detecting gray mold in tomato. Journal of Microbiology, Biotechnology and Food Sciences, e3108. https://doi.org/10.15414/jmbfs.3108

Issue

Section

Food Sciences
Received 2020-05-11
Accepted 2021-04-16
Online Published 2021-05-07