SPONGE-ASSOCIATED FUNGI ISOLATES FROM ANCORINA SP. SHOWED ANTI-CANCER ACTIVITY AGAINST HELA CELL LINES

Authors

  • Abrory Agus Cahya Pramana Dept. of Biochemistry, Faculty of Medicine, Public Health, and Nursing, UGM https://orcid.org/0000-0002-9636-2668
  • Eka Ramadhani Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia https://orcid.org/0000-0002-6085-9998
  • Fajar Priyambada Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia
  • Gian Aditya Pertiwi Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia https://orcid.org/0000-0002-7464-5931
  • R. Aditya Aryandi Setiawibawa Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia https://orcid.org/0000-0001-5111-6765
  • Nastiti Wijayanti Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia https://orcid.org/0000-0001-6483-2816

DOI:

https://doi.org/10.55251/jmbfs.3352

Keywords:

Ancorina sp., Hela cell, SAF KU4, Apoptotic, Cytotoxic

Abstract

Nowadays, natural products from marine life, especially sponge-associated fungi (SAF), have been getting extensive attention as candidates for anti-cancer agents. Although chemotherapy has been widely used as one of the cancer treatments, recently, multidrug resistance as one of its drawbacks has appeared. Our preliminary research successfully isolated 16 fungi isolates from Ancorina sp. habituating Kukup (KU) Beach, Yogyakarta, Indonesia. The ethyl acetate extracts of SAF KU4 fungi grown in enrichment broth media in seawater base either as media-free mycelium or whole medium were analyzed for the cytotoxicity level and the apoptotic effect on the apoptotic effect cervical cancer cell line (HeLa). SAF KU4 was cultured in Wickerham sea-based media and then extracted using ethyl acetate. The IC50 values were measured by MTT assay. IC50 of the media-free extract and whole extract were 158.13 and 283.95 µg/mL, respectively. Ethyl acetate extracts of media-free mycelium and whole extract were moderately cytotoxic but could induce apoptosis in HeLa cell lines.

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Published

2022-08-01

How to Cite

Pramana, A. A. C., Ramadhani, E. ., Priyambada, F., Pertiwi, G. A., Setiawibawa, R. A. A., & Wijayanti, N. (2022). SPONGE-ASSOCIATED FUNGI ISOLATES FROM ANCORINA SP. SHOWED ANTI-CANCER ACTIVITY AGAINST HELA CELL LINES. Journal of Microbiology, Biotechnology and Food Sciences, 12(1), e3352. https://doi.org/10.55251/jmbfs.3352

Issue

Section

Biotechnology
Received 2020-06-29
Accepted 2022-02-09
Online Published 2022-08-01