EXTRACELLULAR ALKALINE PECTINASES PRODUCTION: A REVIEW
Keywords:Alkaline Pectinases, Optimization strategies, Genetic engineering, Fermentation media
Pectinases are most significant enzymes of commercial sector. Among all the commercially produced industrial enzymes, pectinases occupy 25% share in the overall enzyme market. Pectinases have wide applications in various processes such as fruit juice clarification, wastewater remediation and textile and paper industry etc. Pectinase is most suitable environment friendly tool of nature for sustainable development. Although, pectinases alike many other commercial enzymes are facing the imperative of low harvest and yield. Hence, pectinases have been the objective of research to accomplish the commercial scale articulation levels. To find unique physicochemical properties of pectinases often microbial pectinases are utilized among all the natural sources due to ease in production. Present study primarily centers on the different types of recent techniques for the alkaline pectinases production and optimization strategies advancement for high production yield. For achieving higher production of the extracellular alkaline pectinases by utilizing basic fermentation media and simple molecular devices along with genetic engineering approaches are included in the present study. For the improved alkaline pectinase production advanced genetic engineering techniques such as gene cloning, tools of r-DNA technology and cell immobilization technique are discussed.
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Copyright (c) 2022 Pitambri THAKUR, Abhishek Kumar Singh, Manjeet SINGH, Gunjan MUKHERJEE
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