ISOLATION, SCREENING, AND PARTIAL CHARACTERIZATION OF OXALATE OXIDASE ENZYME FROM PSEUDOMONAS STRAIN UNDER INDUCED OXALATE STRESS CONDITION

Authors

  • ANITA PATIL Department of Biotechnology, Sant Gadge Baba Amravati University Amravati (Maharashtra), India
  • HARIPRASAD PAIKRAO ASSISTANT PROFESSOR https://orcid.org/0000-0002-3562-7271
  • RUBINA SHEIKH Department of Biotechnology, Sant Gadge Baba Amravati University Amravati (Maharashtra), India. https://orcid.org/0000-0001-8942-1933
  • LEENA DHAKATE Department of Biotechnology, Sant Gadge Baba Amravati University Amravati (Maharashtra), India. https://orcid.org/0000-0002-8710-6427

DOI:

https://doi.org/10.15414/jmbfs.4174

Keywords:

Oxalate oxidase, Salt stress, Pseudomonas strain, Peroxidase

Abstract

Oxalate oxidase (OxO), a manganese dependent enzyme, is involved in the catalysis of oxalate oxidation to carbon dioxide with the formation of hydrogen peroxide. OxO is present in the cell wall of plants. It increases the resistivity against diseases and external stress. Oxalate is found as waste metabolites in mammals, excess accumulation of oxalate results in hyperoxaluria and urolithiasis in humans. These disorders can be diagnosed by the help of OxO in many analytical methods. The present study is aimed on isolation of OxO enzyme fromĀ  Pseudomonas strain under high salt stress. The OxO enzyme was produced in bulk under selected salt stress. It was partially purified by ammonium sulphate precipitation method, dialysis and ion-exchange chromatography. The OxO enzyme on enzymatic analysis showed potent activity concerning oxalic acid substrate. The microtiter plate analysis confirmed the ability of OxO enzyme purified from Pseudomonas strain in the diagnosis of oxalate-related disorders and in the future could be used to prepare the diagnostic biosensors.

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Published

2021-11-24

How to Cite

PATIL, A. ., PAIKRAO, H., SHEIKH, R., & DHAKATE, L. (2021). ISOLATION, SCREENING, AND PARTIAL CHARACTERIZATION OF OXALATE OXIDASE ENZYME FROM PSEUDOMONAS STRAIN UNDER INDUCED OXALATE STRESS CONDITION. Journal of Microbiology, Biotechnology and Food Sciences, e4174. https://doi.org/10.15414/jmbfs.4174

Issue

Section

Microbiology
Received 2021-01-07
Accepted 2021-11-08
Online Published 2021-11-24