THE THE EFFECTS OF ORIENTIN ON THP-1 CELLS INFECTED BY DENV2

Abstract

Orientin, a flavonoid known for its significant antioxidative properties, has also shown to possess antiviral properties against some viruses. Nevertheless, up to now, there was no report on its ability against dengue virus. This study investigated the effects of orientin against DENV2 infecting human monocyte, THP-1 cells. THP-1 cells infected with DENV2 (3.16×10² TCID₅₀/mL) were treated with a maximum non-toxic dose (MNTD) of orientin (0.053 ± 0.006 µM) for two days. Cytopathic effect (CPE) formation was observed after two days, followed by measurement of the percentage of cell viability using 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The treatment was compared to cells infected with DENV2 only, cells treated with orientin at MNTD only, and control cells. The MNTD of orientin on THP-1 cells were determined to be 0.053 ± 0.006 µM. Treatment of DENV2 infected THP-1 cells with the MNTD of orientin showed CPE formation similar to the THP-1 cells infected with DENV2 only. The MTT assay showed that orientin did not exhibit antiviral activity against DENV2 infecting THP-1 cells. Percentage of cell viability of the orientin treated cells and DENV2-infected cells were not significantly different. Orientin at MNTD does not exhibit antiviral effects on THP-1 cells infected with DENV2.