AUTHENTICITY ANALYSIS OF BEEF MEATBALL ADULTERATION WITH WILD BOAR USING FTIR SPECTROSCOPY COMBINED WITH CHEMOMETRICS

Abstract

The aim of this research to detect and analyze the wild boar in beef meatball using FTIR combined with chemometric. Then, the specific of FTIR region for detecting wild boar in beef meatball in the commercial product. The wild boar meatball was made with different a concentration range between 0-100%. The wild boar meatball was analyzed using FTIR. The FTIR spectrum was processed using chemometrics method like Part Least Square (PLS) and Principal Component Analysis (PCA). The PLS look for the highest of determination coefficient and smaller of root mean square error of calibration (RMSEC) or Root Mean Square Error of Cross-Validation (RMSECV). PCA analysis will distinguish the wild boar meatball and beef meatball product. The result of this research showed that the FTIR spectrum of wild boar and beef in meatball have different characteristics. The result from FTIR spectrum processing showed that specific spectrum of wild boar at wavenumber 999 - 1481 cm-1 combined with 1650 - 1793 cm-1 which is processed at 1st derivative. The PLS resulted from the calibration method has the determination coefficient (R2) value is 0.9991 and RMSEC of 1.028%. While the validation methods resulted are R2 and RMSECV which are 0.9999 and 0.300% respectively. This research showed the meatball from wild boar can be distinguished from beef and the commercial meatballs are not made from them.