PROTECTIVE EFFECT OF CELASTRUS PANICULATUS SEEDS EXTRACT AGAINST LIPOPOLYSACCHARIDE-INDUCED PAW EDEMA IN RATS

Authors

  • Anitha K N Dayanada Sagar University
  • Dr Geetha KM Department of Pharmacology, College of Pharmaceutical Sciences, Dayananda Sagar, University, Kumaraswamy Layout, Bangalore-560078, (Karnataka) India

DOI:

https://doi.org/10.55251/jmbfs.4713

Keywords:

Jyotishmati, nociceptive bioassays, Randall-Selitto test, linoleic acid, 5-HETE

Abstract

The present study was designed to evaluate the effect of Celastrus paniculatus seeds methanolic extract (CPME) against lipopolysaccharide (LPS)-induced paw edema and nociception in rats.  Moreover, in-vitro test for soluble epoxide hydrolase (sEH) inhibition activity of CPME was conducted to study its possible mode of action. 

Celastrus paniculatus seeds were extracted with methanol by cold maceration for 7 days. The obtained dark brown coloured extract was evaluated for preliminary phytochemical screening and subjected for HPTLC analysis. Then the extract was evaluated for its action against LPS-induced paw edema in rats at the dose of 100, 200 and 400 mg/kg body weight by measuring the paw edema, nociceptive bioassays and estimating 5-HETE in serum. In addition, CPME was evaluated for in-vitro sEH inhibition activity.

Administration of CPME attenuates LPS-induced paw edema by 22.85% and serum 5-HETE levels by 9.56% (P<0.001) and elevated pain threshold by 112.19% (P<0.001). The CPME inhibits human, mouse and rat sEH activity with IC50 of 4.60 µg/mL, 4.17 µg/mL and 5.18 µg/mL, respectively. The present study results indicate that CPME has protective effect against LPS-induced inflammation and pain in rats. The possible mode of action is attenuation of 5-HETE levels and sEH inhibition. However, further research is warranted to elucidate and isolate the active constituents from Celastrus paniculatus seeds.

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Published

2022-06-02

How to Cite

K N, A., & Geetha, K. (2022). PROTECTIVE EFFECT OF CELASTRUS PANICULATUS SEEDS EXTRACT AGAINST LIPOPOLYSACCHARIDE-INDUCED PAW EDEMA IN RATS. Journal of Microbiology, Biotechnology and Food Sciences, 11(6), e4713. https://doi.org/10.55251/jmbfs.4713

Issue

Section

Biotechnology
Received 2021-05-07
Accepted 2022-01-20
Online Published 2022-06-02