CONVENTIONAL PRODUCTION OPTIMIZATION OF CYCLODEXTRIN GLUCOSYL TRANSFERASE BY A NOVEL ISOLATE OF BACILLUS SP. PBS1 FROM POTATO RHIZOSPHERE
Keywords:CGTase, Bacillus sp. PBS1, Î²-cyclodextrin, Production optimization
The cyclodextrin glucosyltransferase enzyme (CGTase) is an industrially crucial enzyme for the production of Î²-cyclodextrin (Î²-CD). CGTase has a high propensity to produce a mixture of cyclodextrins (CDs). From the industrial perspective, a CGTase that produces only one type of CD is of critical importance.Â BacillusÂ sp. PBS1Â produced CGTase that converts starch solely into Î²-CD. The isolated strain PBS1 was found to close similarity with alkaliphilicÂ BacillusÂ sp. based on biochemical, morphological, and phylogenetic analysis of its 16s rRNA gene sequencing. The selection and optimization of media ingredients are warranted for the best possible production of Î²-CD. These steps were carried out by conventional optimization strategies. The presence of glucose, maltose, lactose, sucrose, galactose, mannitol, nitrates, urea, metal salts, and K2HPO4 led to the suppression of CGTase production. The improved enzyme production was observed in peptone, soluble starch, magnesium sulfate, and Na2CO3. The organism produces maximum CGTase (93.42 Â± 2.4 U/ml) at 96-hour incubation in the optimized production medium containing 8% starch, 2% peptone, 0.06% MgSO4.7H2O, 0.5% Na2CO3, and having pH of 9.3. The optimization of the medium led to ~16% improvement in CGTase production byÂ BacillusÂ sp. PBS1.
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Copyright (c) 2021 Preetibala Solanki, Pushpendra Awadhiya, Tushar Banerjee
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Online Published 2022-08-01