CONVENTIONAL PRODUCTION OPTIMIZATION OF CYCLODEXTRIN GLUCOSYL TRANSFERASE BY A NOVEL ISOLATE OF BACILLUS SP. PBS1 FROM POTATO RHIZOSPHERE
Keywords:CGTase, Bacillus sp. PBS1, β-cyclodextrin, Production optimization
The cyclodextrin glucosyltransferase enzyme (CGTase) is an industrially crucial enzyme for the production of β-cyclodextrin (β-CD). CGTase has a high propensity to produce a mixture of cyclodextrins (CDs). From the industrial perspective, a CGTase that produces only one type of CD is of critical importance. Bacillus sp. PBS1 produced CGTase that converts starch solely into β-CD. The isolated strain PBS1 was found to close similarity with alkaliphilic Bacillus sp. based on biochemical, morphological, and phylogenetic analysis of its 16s rRNA gene sequencing. The selection and optimization of media ingredients are warranted for the best possible production of β-CD. These steps were carried out by conventional optimization strategies. The presence of glucose, maltose, lactose, sucrose, galactose, mannitol, nitrates, urea, metal salts, and K2HPO4 led to the suppression of CGTase production. The improved enzyme production was observed in peptone, soluble starch, magnesium sulfate, and Na2CO3. The organism produces maximum CGTase (93.42 ± 2.4 U/ml) at 96-hour incubation in the optimized production medium containing 8% starch, 2% peptone, 0.06% MgSO4.7H2O, 0.5% Na2CO3, and having pH of 9.3. The optimization of the medium led to ~16% improvement in CGTase production by Bacillus sp. PBS1.
How to Cite
Copyright (c) 2021 Preetibala Solanki, Pushpendra Awadhiya, Tushar Banerjee
This work is licensed under a Creative Commons Attribution 4.0 International License.
All papers published in the Journal of Microbiology, Biotechnology and Food Sciences are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).
Online Published 2022-04-07