PYOCIN S5 ACTIVITY AGAINST PSEUDOMONAS SYRINGAE PHYTOPATHOGENIC STRAINS

Oleksandr Balko; Lyubov Zelena; Olha Balko; Liliya  Avdeeva

doi:10.55251/jmbfs.5607

Authors

Oleksandr Balko D.K. Zabolotny Institute of Microbiology and Virology https://orcid.org/0000-0003-2635-3464
Lyubov Zelena National Academy of Sciences of Ukraine, D.K. Zabolotny Institute of Microbiology and Virology, Department of Physiology of industrial microorganisms https://orcid.org/0000-0002-5148-1030
Olha Balko Department of Antibiotics, D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine
Liliya Avdeeva Department of Antibiotics, D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine https://orcid.org/0000-0002-8458-444X

DOI:

https://doi.org/10.55251/jmbfs.5607

Keywords:

Pyocin S5, purification, Pseudomonas aeruginosa, antimicrobial activity, Pseudomonas syringae

Abstract

Pseudomonas syringae is the most frequently isolated bacterial plant pathogens. Previously we revealed a number of Pseudomonas aeruginosa strains – producers of bacteriocins (pyocins) with high activity against P. syringae. However, it was unknown, which type of pyocins possesses the activity against P. syringae strains. S5 and S1 pyocin genes were observed in the genome of most active strain–producer P. aeruginosa UCM В-333. Pyocins were induced by nalidixic acid (100 μg/ml). Using DEAE-cellulose chromatography we isolated two bacteriocin groups from initial lysate. Substances from the 10^th fraction had nuclease activity (pyocin S1) and the 27^th fraction - pore-forming activity (pyocin S5). It was revealed that S1 pyocin didn’t lead to growth inhibition of studied bacterial plant pathogens. However pyocin S5 caused appearance of growth inhibition area with diameter 14-20 mm. So, pyocins of S1 and S5 types are active substances in P. aeruginosa UCM В-333 lysates, but only S5 pyocin possess activity against P. syringae strains. fpt A receptor gene was revealed in genomes of all analyzed cultures, including used phytopathogenic strains. This fact can explain wide spectrum of pyocin S5 activity. It is suggested that pyocin S5 can be a potential biocontrol agent against phytopathogenic P. syringae strains.