PRODUCTION OF POLYCLONAL ANTIBODY AGAINST THE OUTER MEMBRANE PROTEIN Omp48 OF Aeromonas hydrophila

Authors

DOI:

https://doi.org/10.55251/jmbfs.5816

Keywords:

A. hydrophila, Outer membrane protein, Omp48, polyclonal antibody

Abstract

Aeromonas hydrophila is one of the main pathogens in fish, causing a huge economic loss every year. Since outer membrane protein Omp48 had been demonstrated to be immunogenic in fish, it has gained attention as a potential vaccine candidate to protect fish from A. hydrophila.  In this study, we produced a polyclonal antibody against Omp48, which would be necessary for further studies on this protein. To reach this aim, 6xHis-tagged Omp48 was expressed in a recombinant E. coli strain and purified by a nickel affinity chromatography column, which yielded a purity of 97.3 ± 1.4%. The purified Omp48 was injected into rabbits in order to produce polyclonal anti-Omp48 antibody. Subsequently, we analyzed the obtained antiserum, which was demonstrated to have high specificity and sensitivity to the Omp48 protein by Western blot and ELISA, with the titer of 1:1,280,000. In addition, we also showed that the antiserum could be used to detect Omp48 in immunofluorescence staining.

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Published

2022-08-01

How to Cite

Thi, H. R., Nguyen, V. H., Do, N. T. T., Dang Thi, P. T., & Nguyen, T. (2022). PRODUCTION OF POLYCLONAL ANTIBODY AGAINST THE OUTER MEMBRANE PROTEIN Omp48 OF Aeromonas hydrophila. Journal of Microbiology, Biotechnology and Food Sciences, 12(1), e5816. https://doi.org/10.55251/jmbfs.5816
Received 2022-02-06
Accepted 2022-05-05
Online Published 2022-08-01