PRODUCTION AND OPTIMIZATION OF GROWTH CONDITIONS FOR INVERTASE ENZYME BY ASPERGILLUS SP., IN SOLID STATE FERMENTATION (SSF) USING PAPAYA PEEL AS SUBSTRATE

Authors

  • Brindha Chelliappan
  • K. Madhanasundareswari

Keywords:

Invertase, Aspergillus sp., papaya peel substrate, solid state fermentation (SSF), optimization

Abstract

Invertase enzymes are produced mainly by plants, some filamentous fungi, yeast and many other microorganisms which finds applications in food industries, confectionaries, pharmaceuticals, etc., The present work deals with the production of Invertase by Aspergillus sp., isolated from various soil samples in solid state fermentation using papaya peel waste as substrate. Enzyme activity was checked using Fehling’s reagent and assay was carried out by DNSA method. The results of optimized conditions showed that the invertase activity was high in the SSF using papaya peel as substrate, incubated for 6 days at temperature of 35°C, pH 7, with 2.25gms/100ml of Ammonium nitrate as nitrogen source and 10gms/100ml of sucrose as carbon source. Hence the agro wastes from industries can be recycled by using it as substrate in SSF for high invertase enzyme production which finds applications in many fields.

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Published

2013-12-01

How to Cite

Chelliappan, B., & Madhanasundareswari, K. (2013). PRODUCTION AND OPTIMIZATION OF GROWTH CONDITIONS FOR INVERTASE ENZYME BY ASPERGILLUS SP., IN SOLID STATE FERMENTATION (SSF) USING PAPAYA PEEL AS SUBSTRATE. Journal of Microbiology, Biotechnology and Food Sciences, 3(3), 266–269. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7033

Issue

Vol. 3 No. 3 (2013): December - January

Section

Microbiology

License

Copyright (c) 2013 Brindha Chelliappan, K. Madhanasundareswari

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Journal of Microbiology, Biotechnology and Food Sciences  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).