APOPTIC ACTIVITY OF CHICKEN ANEMIA VIRUS VP3 GENE CLONED IN REPLICASE BASED EUKARYOTIC VECTOR

Authors

  • Priyanka Pal
  • Kusum Agarwal
  • Anant Rai
  • Usha Tiwari

Keywords:

VP3 gene, apoptin, apoptosis, replicase vector, antitumor

Abstract

CAV is one of the smallest avian viruses; it is 23–25 nm in size, icosahedral in shape and non-enveloped, having a 2.3 kb, circular, single-stranded, negative sense DNA genome. The genome encodes three viral proteins VP1, VP2 and VP3, that are transcribed from a single major transcript of 2.0 kb. The apoptosis inducing potential of CAV-VP3 (apoptin) was studied in HeLa cell line. The study confirmed that VP3 induced apoptosis in HeLa cells, which was confirmed by demonstrating the characteristics changes of apoptosis which include nuclear condensation, DNA fragmentation by DNA laddering assay, plasma membrane alteration by annexin-V binding assay and Caspase 3. The objective of our work was to study the apoptic activity of apoptin in HeLa cell line.

Downloads

Download data is not yet available.

Downloads

Published

2013-12-01

How to Cite

Pal, P., Agarwal, K., Rai, A., & Tiwari, U. (2013). APOPTIC ACTIVITY OF CHICKEN ANEMIA VIRUS VP3 GENE CLONED IN REPLICASE BASED EUKARYOTIC VECTOR. Journal of Microbiology, Biotechnology and Food Sciences, 3(3), 240–242. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7040

Issue

Vol. 3 No. 3 (2013): December - January

Section

Biotechnology

License

Copyright (c) 2013 Priyanka Pal, Kusum Agarwal, Anant Rai, Usha Tiwari

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Journal of Microbiology, Biotechnology and Food Sciences  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).