PARTIAL CHARACTERIZATION OF ENZYMATIC ACTIVITIES PRODUCED BY A WILD STRAIN OF A. NIGER

Authors

  • María Martos
  • Alicia Baumann
  • Nancy Cruz
  • Roque Hours
  • Oscar Garro

Keywords:

Aspergillus niger, pecinolytic enzymes, polygalacturonase

Abstract

Aspergillus niger, isolated from decay citrus peels in the province of Misiones, was able to produce pectinases by submerged fermentation. The enzymatic extract exhibited polygalacturonase, pectinesterase and lyase activities. Others enzymes capable of degrading cell wall polymers were also detected in the enzymatic extract such as cellulases and xylanases. Polygalacturonase was an endo-polygalacturonase. The enzyme exhibited a maximal activity at pH range between 4.5 to 5.0, was stable in the pH range from 2.5 to 5.5 and remained unchanged when was incubated at temperatures lower than 50 ºC. The fungi produced three PG isoenzymes. The enzymatic extract was able to clarify apple juice.
The results observed make the pectinolytic enzymes produced by A. niger appropriate for future application in fruit juice processing industries.

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Published

2012-12-03

How to Cite

Martos, M., Baumann, A., Cruz, N., Hours, R., & Garro, O. (2012). PARTIAL CHARACTERIZATION OF ENZYMATIC ACTIVITIES PRODUCED BY A WILD STRAIN OF A. NIGER. Journal of Microbiology, Biotechnology and Food Sciences, 2(3), 917–932. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7135

Issue

Vol. 2 No. 3 (2012): December - January

Section

Microbiology

License

Copyright (c) 2012 María Martos, Alicia Baumann, Nancy Cruz, Roque Hours, Oscar Garro

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Journal of Microbiology, Biotechnology and Food Sciences  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).