COMPARATIVE EVALUTION OF CEPHALOSPORIN-C PRODUCTION IN SOLID STATE FERMENTATION AND SUBMERGED LIQUID CULTURE

Abstract

The advantages of solid state fermentation (SSF) utilization in producing enzymes & secondary metabolites have been shown, whereas, submerged liquid fermentation (SLF) condition has the major usage in industrial production of antibiotics. As an antibiotic of B-lactams group, cephalosporin-C (CPC) is indicated due to its wide effect and broad convention in treatment of infectious diseases. Regarding industrial production of CPC regularly done in SLF condition, we compared CPC production sum in SLF and SSF conditions.
In this analysis, A. chrysogenum was employed, which was inoculated to SLF and SSF, while internal fermenter conditions were totally under control. After extraction of CPC, productions in two states of SLF and SSF were compared using the cylinder plate method.
According to Antibiotic assay and production amount comparison, results expressed a ratio of development of production in SSF conditions to SLF conditions. Regarding previous studies on a solid state fermenter and its advantages, in this study, convenience of SSF conditions compared to SLF conditions was experimented. Also mentioning that maintaining the condition of solid state fermenter is more comfortable and practical than liquid state fermenter, using a solid based fermenter to produce antibiotics, especially CPC, can be appropriate. Considering appropriate control conditions of SSF to produce secondary metabolites, decrease in expenses, and increase of production, taking advantage of it in order to increase production parallel to modern methods, such as genetically manipulating CPC producing microorganisms are recommended to pharmacological industries. Also, to make this method applicable, further studies in industrial criterion seem necessary.