LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) FOR THE DETECTION OF SALMONELLA SPP. ISOLATED FROM DIFFERENT FOOD TYPES

Authors

  • Panos G. Ziros
  • Petros A. Kokkinos
  • Kostas Papanotas
  • Apostolos Vantarakis

Keywords:

Loop-Mediated Isothermal Amplification, Salmonella, Detection, Food Matrices

Abstract

The objective of this study was the application and evaluation of a loop-mediated isothermal amplification (LAMP) method for the detection of Salmonella spp. strains isolated from food samples. Salmonella specific invA gene sequences (50 strains, 15 serotypes) were amplified at 65oC in 60 min. All of the strains of Salmonella subsp. Enterica were shown to be positive using the LAMP reaction assay, whereas, all other bacteria, virus and yeasts tested in this study were negative. LAMP products could be visually detected under day light or ultraviolet light, while the specific amplification of the DNA of Salmonella strains generated ladder-like pattern bands on agarose gel. LAMP is suitable for the sensitive, rapid, and inexpensive detection of Salmonella spp. in food analytical laboratories.

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Published

2012-08-01

How to Cite

G. Ziros, P., A. Kokkinos, P., Papanotas, K., & Vantarakis, A. (2012). LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) FOR THE DETECTION OF SALMONELLA SPP. ISOLATED FROM DIFFERENT FOOD TYPES . Journal of Microbiology, Biotechnology and Food Sciences, 2(1), 152–161. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7194

Issue

Vol. 2 No. 1 (2012): August - September

Section

Microbiology

License

Copyright (c) 2012 Panos G. Ziros, Petros A. Kokkinos, Kostas Papanotas, Apostolos Vantarakis

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Journal of Microbiology, Biotechnology and Food Sciences  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).