ANTIBACTERIAL AND CYTOTOXIC ACTIVITY OF BACILLUS METHYLOTROPHICUS-SCS2012 ISOLATED FROM SOIL

Authors

  • Subed Chandra Dev Sharma
  • Mohammad Shariar Shovon
  • M. G. Sarowar Jahan
  • A.K.M. Asaduzzaman
  • Md. Ajijur Rahman
  • Kamal Krishna Biswas
  • N. Abe
  • Narayan Roy

Keywords:

Bacillus methylotrophycus-SCS2012, antibacterial activity, minimum inhibitory concentration, cytotoxicity

Abstract

The strain Bacillus methylotrophycus-SCS2012 (Bacillus sp. SCS2012) identified by 16S rRNA gene sequencing was isolated from soil. Ethyl acetate extract of B. methylotrophycus-SCS2012 showed antibacterial activity against both Gram positive and Gram negative bacteria. The crude metabolite extracted from B. methylotrophycus-SCS2012 exhibited strong antibacterial activity against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae with the zone of inhibition range 28.33 to 32.16 mm at 100μg/disc. Minimum inhibitory concentration (MIC) of the extract against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae was found to be 156, 156, 312, 312 and 625µg/ml respectively. The ethyl acetate extract also exhibited cytotoxic effects on brine shrimp lethality bioassay with LC50 value of 10.78 ï­g/ml. Thus the ethyl acetate extract of Bacillus methylotrophycus-SCS2012 has broad spectrum activity with moderate cytotoxicity.

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Published

2013-02-01

How to Cite

Chandra Dev Sharma, S., Shariar Shovon, M., G. Sarowar Jahan, M., Asaduzzaman, A., Ajijur Rahman, M., Krishna Biswas, K., Abe, N., & Roy, N. (2013). ANTIBACTERIAL AND CYTOTOXIC ACTIVITY OF BACILLUS METHYLOTROPHICUS-SCS2012 ISOLATED FROM SOIL. Journal of Microbiology, Biotechnology and Food Sciences, 2(4), 2293–2307. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7493

Issue

Vol. 2 No. 4 (2013): February - March

Section

Microbiology

License

Copyright (c) 2013 Subed Chandra Dev Sharma, Mohammad Shariar Shovon, M. G. Sarowar Jahan, A.K.M. Asaduzzaman, Md. Ajijur Rahman, Kamal Krishna Biswas, N. Abe, Narayan Roy

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